Exploring the Turbot pcsk2 gene
(a) Find the Turbot pcsk2 (proprotein convertase subtilisin/kexin type 2) gene, and go to the Gene tab.
- On which chromosome and which strand of the genome is this gene located?
- How many transcripts (splice variants) are there and how many are protein coding?
- How long is the protein encoded by ENSSMAT00000034168.2?
- What are some functions of pcsk2 according to the Gene Ontology consortium?
(b) In the transcript table, click on the transcript ID for pcsk2-201, and go to the Transcript tab.
- How many exons does it have?
- Are any of the exons completely or partially untranslated?
(c) Is there any supporting evidence present for ENSSMAT00000034168.2?
(a) Go to the Ensembl homepage.
Select Search: Turbot and type pcsk2. Click Go.
Click on the Ensembl ID ENSSMAG00000020650.
- Chromosome 15 on the reverse strand.
- Ensembl has five protein coding transcripts annotated for this gene.
- It codes for a protein of 629 amino acids
Click on GO:molecular function to see that serine-type endopeptidase activity and serine-type peptidase activity are associated with pcsk2.
(b) Click on ENSSMAT00000034168.2.
It has thirteen exons. This is shown in the Transcript summary or in the left hand side menu Exons.
Click on the Exons link in this side menu.
Exons 1 and 13 are partially untranslated (UTR sequence is shown in orange). You can also see this in the cDNA view if you click on the cDNA link in the left side menu.
(c) Click on Supporting evidence in the left menu.
The image shows Transcript supporting evidence which comes from alignments used to build the transcript model and Exon supporting evidence which is alignments from the Ensembl pipeline that support the exons.
