Finding orthologous genes for disease resistance gene in Arabidopsis thaliana
Resistance to the leaf rust delivered by SH3 factor(s) is well-grounded as specially durable. In 2023, Paula Cristina da Silva Angelo et al (https://doi.org/10.1016/j.pmpp.2023.102111) reported that the Arabidopsis thaliana gene AT1G50180 is an important gene in the SH3 locus conferring diseae resistance.
Search Ensembl for the gene AT1G50180 in Arabidopsis thaliana (Thale cress) TAIR10.
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Find homologous genes for AT1G50180 .
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How many results can you see? Which species gene is closest to AT1G50180 when you consider protein similarity and coverage?
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Download the protein sequence for transcript AT1G50180.1.
Go to Ensembl Select Arabidopsis thaliana (Thale cress) TAIR10 from the species selector app and search for AT1G50180. Click Find a gene on the right-hand side panel and enter the gene ID, click on the gene ID AT1G50180 and then click on the Entity viewer icon.
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Click on the Gene relationships tab on the top. The Gene relationships panel shows a table that lists genes homologous to your gene of interest.
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Homologous genes share a common ancestor, being separated or ‘split’ by a speciation event or a gene duplication event. The % Protein similarity is the percentage of identical amino acid residues aligned against each other. Whereas, the % coverage is the percent of query which is in local alignment with the respective reference.
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Click on the Down arrowhead button on the right-hand side. Select cDNA from under All transcripts and finalise your export by clicking on the Download button.
