Exploring the zebrafish prkci gene
Search for the zebrafish gene prkci.
(a) What GO: biological process terms are associated with the prkci gene?
(b) Go to the transcript tab for the only protein coding transcript. How many exons does it have? Which one is the longest? How much of that is coding?
(c) How many different domain prediction methods predict a PB1 domain? Where in the protein is this domain?
(a) Go to the Ensembl homepage (http://www.ensembl.org).
Select Search: Zebrafish and type prkci. Click Go. Click on the gene link ENSDARG00000021225.
Click on GO: biological process in the side menu. Protein phosphorylation is listed as GO: Biological process associated with this gene.
(b) Click on the transcript ENSDART00000015723.8. Click on Exons in the left hand menu. There are eighteen exons. Exon 18 is longest with 1,596 bp, of which around 95 are coding.
(c) Click on either Protein Summary or Domains & features in the left hand menu to see graphically or as a table respectively. The PB1 domain is predicted by SMART, Pfam and PROSITE. It is towards the N-terminus of the protein. Clicking on the predicted PB1 domain will open a pop-up window that shows you the amino acid coordinates for the predicted domain.
