Finding a Triticum aestivum gene

Search for Oxygen evolving enhancer protein from the Ensembl Plants homepage and narrow down your search to Triticum aestivum. How many genes are there with this name in wheat? Why do you think this is? What chromosomes are they on?
Go to the gene on chromosome 2B. How many protein-coding transcripts does this gene have? What is a “canonical transcript”?
Click on the canonical transcript. How many exons does this transcript have? Export the protein sequence of this transcript in the FASTA format.

Start at the Ensembl Plants homepage. Choose Triticum aestivum from the species drop-down, type Oxygen evolving enhancer protein into the search box then click Go.

There are two genes named TraesCS2D02G248400 and TraesCS2B02G270300. This is because of the hybridisations in wheat’s evolutionary history. You can see that the two genes occur on chromosomes 2B and 2D.
Click on the gene on chromosome 2B to go to the Gene tab. If the transcript table is hidden, click on Show transcript table to see it.

There are 2 protein coding transcripts.

Mouse over the Ensembl Canonical flag in the transcripts table to find a description.

The Ensembl canonical transcript is a single transcript chosen for each gene in each species. It is the most highly conserved, most highly expressed, has the longest coding sequence and is represented in other key resources (e.g. NCBI, UniProt)
Click on TraesCS2B02G270300.2 in the transcript table. You can find the number of exons in the summary description at the top of the Summary page, or you can count the number of boxes (boxes represent exons, lines represent introns) in the Summary diagram.

TraesCS2B02G270300.2 has 2 exons.

Go to Sequence: Protein In the left-hand panel.

Click on the green Download sequence button above the protein sequence. Select FASTA from the drop-down in the pop-up menu and download the sequence to your local machine.