Exploring the FUS3 gene in Coffea canephora (Robusta coffee)
The FUS3 gene is a known master regulator of somatic embryogenesis, an important factor in stable genetic transformation and successful plant regeneration of coffee trees expressing the Bacillus thuringiensis (Bt) toxin Cry10Aa to induce Coffee Berry Borer (CBB) resistance.
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Find the Coffea canephora FUS3 gene on Ensembl Plants. On which chromosome and which strand of the genome is this gene located?
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Where in the cell is the FUS3 protein located?
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What is the source of the assigned gene description?
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How long is its transcript (in bp)? How long is the protein it encodes? How many exons does it have? Are any of the exons completely or partially untranslated?
- Go to the Ensembl Plants homepage (http://plants.ensembl.org/). Select C. canephora from the species list and type
FUS3in the search box. Click Go and click on the gene ID GSCOC_T00019208001. You can find the strand orientation and the location under Summary in the Gene tab.The C. canephora FUS3 gene is located on chromosome 7 on the forward strand.
- Click on GO: Cellular component in the left-hand panel.
The protein is located in the nucleus.
- Click on Summary in the side menu.
The gene description is Projected from Arabidopsis thaliana (AT3G26790) by UniProtKB/Swiss-Prot;Acc:Q9LW31.
- Click on Show transcript table.
The transcript is 1038 bp and the length of the encoded protein is 279 amino acids.
Click on the transcript ID CDP16731 in the transcript table. You can find the number of exons in under in the summary information at the top of the page.
It has 7 exons.
Click on Sequence: Exons in the left-hand panel.
The last exon is partially untranslated (sequence shown in orange). This can also been seen from the fact that in the transcript diagrams on the Gene Summary and Transcript Summary pages the boxes representing the last exon is partially unfilled.
