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Exploring a bacterial gene in Clostridium sporogenes

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Exploring a bacterial gene in Clostridium sporogenes

Start in Ensembl Bacteria and search for the rpoS gene in Borreliella burgdorferi B31 (GCA_000008685) genome.

  1. What is the gene ID for rpoS? What does this gene code for?

  2. Go to the transcript tab. How long is the transcript? How long is the protein?

  3. What domains can be found in the protein product of this transcript? How many different domain prediction methods agree with each of these domains?

  1. From the Ensembl Bacteria homepage, enter Borreliella burgdorferi B31 (GCA_000008685) in the species search bar. In the species information page, search for rpoS and click on the gene ID BB_0771 which will open the Summary display on the Gene tab..

    The gene ID is BB_0771. The gene codes for an RNA polymerase sigma factor (rpoS).

  2. Switch to the Transcript tab and go to the Summary display. You can find the length under Statistics underneath the transcript image.

    The glnA transcript is 1,899 bp and the protein is 632 aa in length.

  3. Click on either Protein Summary or Domains & features in the left hand menu to see graphically or as a table respectively.

    6 protein domains were found. All of them predict a glutamine synthetase domain.